Question

Start with the temperature at 25 ºC and the pH at 7. Do not vary these parameters while testing for substrate (Initial Lactose) concentration, as they are environmental controls. Substrate (Initial Lactose) concentration is in mg/dL.

Vary the substrate (Initial Lactose) concentration by 250 mg/dL, starting with 0 mg/dL.
Click the green ‘Run’ button.
After the experiment runs, click the graph between 2 and 4 minutes and move it until the time underneath the graph shows ‘Minutes: 3’ and hold it there.

Answer 1

The results show that the increase in the substrate concentration is increasing the rate of the reaction. We can see that the reaction rate has increased to 2.88mM/min. After this even if we increase the substrate concentration