Answer:
The correct answer is c.
Step-by-step explanation:
Polymerase Chain Reaction (PCR) technology is an in vitro enzymatic method that allows the amplification of a specific DNA sequence, that is, it is the DNA amplification technique by means of the polymerase chain reaction (PCR) is a technique that consists of the in vitro amplification of a specific DNA fragment. To carry out the amplification experiment, it is necessary to know, at least partially, the sequence of the fragment to be amplified (a gene, a part of a gene, a non-coding region, ...). Basically, it is about replicating the same DNA fragment over and over again and, for this, we must do in vitro what cells do in vivo to replicate their DNA. Generally, PCR begins with the denaturation or separation of the DNA double helix by heating the sample to a temperature between 94 and 96 ° C to break the hydrogen bonds that joined them, in this way each chain remains as a template for the synthesis of a new complementary strand of DNA.