Question

Which one of the following statements is wrong with respect to separation of DNA fragments on gel electrophoresis?

4 points
a. Gel electrophoresis is employed to check the progression of a restriction enzyme digestion.
b. The DNA fragments move towards cathode under electric field through the matrix.
c. The smaller DNA fragments moves farther.
d. The commonly used matrix is agarose gel.​

Answer 1

b. The DNA fragments move towards cathode under electric field through the matrix.

Step-by-step explanation:

Gel electrophoresis is a molecular biology technique used to separate DNA fragments based on size using electric current. The electric current passes through a GEL (agarose gel is commonly used) matrix. Due to the fact that DNA is negatively charged, they tend to move towards a positively charged electrode called ANODE.

However, the smaller the size of the DNA fragment, the faster and farther it moves along the matrix. Gel electrophoresis is indeed a technique used to check how progressive a restriction enzyme has been able to digest DNA molecule into fragments.