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The upper and lower chambers of the heart are separated by atria ventricles valves septa
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The upper and lower chambers of the heart are separated by

atria
ventricles
valves
septa

User Nikwin
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The upper chambers are called the left and right atria, and the lower chambers are called the left and right ventricles. A wall of muscle called the septum separates the left and right atria and the left and right ventricles.
User Hourglasser
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