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Why do gram negative bacteria lose the primary stain during decolorization with ethanol?.

User Ansshkki
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Answer:

Conversely, the the outer membrane of Gram negative bacteria is degraded and the thinner peptidoglycan layer of Gram negative cells is unable to retain the crystal violet-iodine complex and the color is lost. ... However, the decolorized Gram negative cells are stained red.

User Liran Funaro
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