Answer:
B. Speciation has occurred because breeding between the offspring and original populations would not be possible.
Step-by-step explanation:
Speciation has occurred in the species due to no breeding between the offspring and original populations because the offspring and original species has different number of chromosomes in their DNA. Due to this difference in the chromosome number in the DNA, there is no chance of breeding between offspring and original species. Speciation is the evolutionary process by which populations of organism evolve with the passage of time and become different from the original species.