Question

What is the difference between pcr and sanger sequencing with regard to the materials needed to perform these reactions?.

Answer 1

Answer: The primary difference is the addition of ddNTPs in Sanger Sequencing

Step-by-step explanation:

Sanger sequencing requires a DNA template, a sequencing primer, a thermostable DNA polymerase, nucleotides (dNTPs), dideoxynucleotides (ddNTPs), and buffer.

The various components required for PCR include a DNA template, DNA primers, dNTPs, and DNA polymerase.

ddNTPS lack a 3' -OH causing elongation termination which is necessary for Sanger sequencing.