Question

When preparing a solution in a cuvette, make sure to handle the cuvette by the __________ and avoid touching the ____________ then, ____________ the cuvette before placing it in the spectrophotometer.

Answer 1

When using a cuvette in a spectrophotometer, handle it by the top, avoid touching the optical surfaces, and rinse it with distilled water to prevent contamination and ensure accurate readings.

Step-by-step explanation:

When preparing a solution in a cuvette, make sure to handle the cuvette by the top and avoid touching the optical surfaces; then, rinse the cuvette before placing it in the spectrophotometer. Handling the cuvette by the top ensures that oils from your fingers do not smudge the optical surfaces, which could interfere with the spectrophotometer's readings. Rinsing the cuvette with distilled water, as mentioned in the provided information, is crucial in maintaining sample purity and preventing contamination that could alter the results. Remember to always follow lab safety protocols and use equipment such as micropipettes and UV/vis spectrophotometers with care.

Answer 2

Answer: Textured sides, smooth sides, wipe down.