Question

30) In a dideoxy chain-termination method, you added dideoxy cytosine only instead of dNTPs. What would be the outcome of your sequencing reaction? a) They will all be the same size and end in a G b) They will be of different sizes, all ending in a G c) No product will be formed d) They will be of different sizes, all ending in a C e) They will all be the same size and end in a C

Answer 1

If you add only cytosines instead of dNTPs, this means hat the sequencing would stop after tfilling all the terminal cytosines because it cannot add the other nucleotides needed, so you would end with fragments of different sizes, all of them ending in Cytosine (option d would be correct).