Question

Summarize at least 2 advantages of CRISPR.

Answer 1

Answer: See Below

Step-by-step explanation:

1. CRISPR utilizes a guide RNA (gRNA) that directs the function of a CRISPR protein effector to a specific target gene of choice, providing a versatile programmable platform. The targeting sequence of the gRNA is ~20bp therefore off-target genome editing is less common making it advantageous over most other restriction endonucleases.

2. CRISPR has increased versatility in many aspects and allows for a wide variety of applications in terms of genome editing. For example, The nickase version of the Cas protein induces only a single stranded cut thus opening up opportunities for altered editing. The Cas12a or Cpf1 version of the Cas protein cuts to form 5' sticky ends. And finally for the last example, the Cas protein has been bound to a reverse transcriptase and has shown efficacy in a technique called "Prime Editing" which has an altered gRNA that provides a primer binding site and template for full base insertions, deletions, and combinations of the two.

Answer 2

CRISPR is simple and powerful compared to the rest of technologies used for editing genome. It is simple because it has the ability to point the definite DNA sequences which is facilitated by an easy to synthesize regulated strand of RNA. It is powerful because it let on an immediate target and remove and improve all deterioted DNA sequence present in a gene.