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Metagenomics (check all that apply): Group of answer choices Can leverage Next Generation Sequencing technology to identify and characterize organisms. Rarely finds previously unknown microorganisms. Has resources to support analysis at the DOE-JGI site. Can identify microbiologic organisms without traditional isolation and culturing of individual organisms.

User Ingo Steinke
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Final answer:

Metagenomics is the study of collective genomes of multiple species interacting in an environmental niche, using Next Generation Sequencing to identify and characterize organisms without traditional culturing. Contrary to the claim, it often identifies previously unknown microorganisms and has robust resources, such as the DOE-JGI site.

Step-by-step explanation:

Metagenomics can be defined as the study of the collective genomes of multiple species that grow and interact within an environmental niche. This field of study can significantly leverage Next Generation Sequencing technology to identify and characterize organisms that are part of a microbial community. Unlike traditional methods that required culturing microorganisms in isolation, which is often challenging due to resistance to artificial growing conditions, metagenomics can identify microbiologic organisms directly from their natural environment without the need for culturing. This approach not only allows for the identification of new species more rapidly but also provides insights into the effects of environmental factors like pollutants on microbial communities.

It is not true that metagenomics rarely finds previously unknown microorganisms; in fact, it often uncovers species that have not been identified before. Moreover, metagenomics has ample resources available for analysis, including those at powerful facilities like the DOE-JGI site, to support and enhance the abilities of researchers in this field. Therefore, the study of metagenomics is a transformative area of research in microbial genomics with vast applications including environmental monitoring, creation of new biofuels, and understanding the human microbiome and other complex microbial ecosystems.

User Phileas
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Answer:

- Can leverage Next Generation Sequencing technology to identify and characterize organisms

- Has resources to support analysis at the DOE-JGI site.

- Can identify microbiologic organisms without traditional isolation and culturing of individual organisms.

Step-by-step explanation:

Metagenomics can be defined as the study of whole genomes of biological communities recovered from environmental samples. This genomic field has enabled the discovery of new species (microorganisms) and their effects on the environment. Next-Generation Sequencing (NGS) technologies allow to obtain huge amounts of genomic data, which has been a limitation in genomics and metagenomics. Metagenomic NGS (mNGS) is a technique used for sequencing nucleic acids present in a biological sample containing mixed populations of microorganisms. Finally, the Department of Energy Joint Genome Institute (DOE JGI) is a referent in metagenomic analysis, especially in genome assembly data obtained from microbial communities. This Science User Facility has developed a series of bioinformatics tools and databases in order to analyze metagenomic information.

User Jim Ierley
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