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21 votes
One method for predicting the average temperature (in ºC) at which a short DNA molecule binds with its complement is to calculate a melting temperature (Tm) using the following formula. Tm = 4 (G + C) + 2 (A + T) Using the formula, CALCULATE the predicted Tm for the short single-stranded DNA molecule shown below? 5’-ATGCGCGACGCGATTTGAC-3’

User PermGenError
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1 Answer

26 votes
26 votes
Tm = 4 (11) + 2 (8) = 60 degrees Celsius
User Alessandro Verona
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