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Primers have been designed to bind to the sections of DNA shown below in three individuals. Based on the image, which of the following statements is true?

A. The primers are surrounding an STR region and will result in segments of different lengths.
B. The primers are surrounding an STR region and will result in segments of the same length.
C. The primers are not surrounding an STR region and will result in segments of different lengths.
D. The primers are not surrounding an STR region and will result in segments of the same length.

User Federkun
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2 Answers

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22 votes

Final answer:

Without the provided DNA sequences and PCR primer locations, it is impossible to determine whether the primers surround an STR region or not, and whether they will produce segments of different lengths. Understanding primer design in PCR is crucial for amplifying specific DNA sequences, including STR regions which can result in variable length products if present.

Step-by-step explanation:

The student's question pertains to the use of primers designed to bind to specific sections of DNA in PCR amplifications. Primers are short DNA fragments that are complementary to the DNA template and are crucial for the initiation of DNA synthesis in PCR. Primer design is critical and depending on where they bind, they can amplify different parts of the DNA, including Single Tandem Repeats (STRs).

Given the incomplete information about the exact sequences of DNA shown below and how the primers bind, it is challenging to determine the exact length of segments that will result from the PCR. However, if primers surround an STR region, it typically leads to segments of variable length because STR regions consist of repeating sequences that can vary in number among individuals. Conversely, if the primers bind outside of STR regions, the resulting segments would be of the same length, assuming no other variations exists in the sequences being amplified. Without seeing the actual sequences and primer locations, the answer cannot be confidently pinpointed.

User Dellon
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19 votes
19 votes
Primers have been designed to bind to the sections of DNA shown below in three individuals. Based on the image, which of the following statements is true?
A. The primers are surrounding an STR region and will result in segments of different lengths.
B. The primers are surrounding an STR region and will result in segments of the same length.
C. The primers are not surrounding an STR region and will result in segments of different lengths.
D. The primers are not surrounding an STR region and will result in segments of the same length.
User Rob Porter
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3.1k points