For the diffraction grating, the slits spacing should be comparable to the wavelength of length incident on the grating to observe the phenomenon of diffraction.
The wavelength of light considered for the for diffraction ranges from,
By simplifying the given value in meters,
As the value of 1000 nm fall in this range and thus, considered as most reasonable slit spacing.
Hence, option 2 (1000 nm) is the correct answer.