Question

3. How might the changes that you identified affect the protein’s structure?Example: The change would likely have no effect. Neither valine and isoleucine would promote hydrogen bonding with water, because their R groups are nonpolar and thus hydrophobic.Changes spotted in the previous question:1. Valine changed for Isoleucine: there was not a change in the chemical properties, because both amino acids have non-polar chains2. Valine changed for Alanine: there was not a change in the chemical properties, because both amino acids have non-polar chains3. Glutamate changed for Alanine: there was a change because the aminoacidic chain lost their electronegativity4. Glutamate changed for Aspartate: there was not a change because the aminoacidic chain kept it's electronegativity.1) _______________________________________________________________2) _______________________________________________________________3) _______________________________________________________________4) _______________________________________________________________

Answer 1

In the previous exercise we spotted that in the sequences there were at least four changes in the aminoacidic sequences:

1. Valine changed for Isoleucine: as indicated by the very exercise, there wouldn't be a negative effect, because both amino acids have hydrophobic radical groups.

2. Valine changed for Alanine: like in the previous case, there wouldn't be a negative effect, because both amino acids have hydrophobic radical groups.

3. Glutamate changed for Alanine: there would be a change in the protein structure because Glutamate can form hydrogen bonds, and Alanine doesn't.

4. Glutamate changed for Aspartate: there wouldn't be a change in the protein structure because both amino acids can form hydrogen bonds.