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Compound light microscopes and transmission/transmitted light microscopes produce an image based on light transmission through the sample. Explain how the fluorescence microscope layout differs from that of transmission microscopes. Include the source of the light visualized in fluorescence microscopy in your answer.

User Yasarui
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The fluorescence microscope differs from the compound light, and transmitted light, not so much because of the structure, but instead because of how light is used to generate an image/ see the sample.

In the fluorescence microscope, the light does not go through the sample per se, instead, a filter is needed, it works with a higher wavelength, in this case, the light rays go to the sample and excite the species marked with the fluorescence substance, that is to say, the sample must be previously treated so it can absorb light and re-emitted as fluorescence (electromagnetic radiation) therefore creating an image, of light. This is possible due to the proprieties of some molecules, such as fluorochromes.

User AntMan
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