Question

You are tasked with making a series of Furosemide calibration standards for analysis by HPLC with fluorescence detection.

(a) Given Furosemide has a molecular weight of 330.7 g/mol, what weight of furosemide would you need to make a stock solution of 0.001 M (10-3 M) concentration ?

(b) How would you then make up a series of standards from this stock solution, of concentrations 10-4, 10-5, 10-6, 10-7 and 10-8 M Furosemide?

Answer 1

(a) To make a stock solution of 0.001 M concentration, you would need to weigh out 33.07 mg of furosemide.

(b) To make up a series of standards from the stock solution, you would first dilute the stock solution with water to make a working solution of 10-4 M concentration. You would then take 1 mL of the working solution and dilute it with water to make a 10-5 M concentration standard, and so on, until you have standards of 10-4, 10-5, 10-6, 10-7, and 10-8 M concentrations.