Question

Cell biologists studying the mobility of plasma membrane proteins fused mouse and human cells together, creating a heterokaryon. They then examined the localization of mouse and human proteins over time. Initially the mouse and human proteins were restricted to separate halves of the heterokaryon. Over time the mouse and human proteins mixed, with each protein eventually being present over the entire cell surface, consistent with proteins being able to move within the plane of the membrane. What techniques could have been used to examine the locations of the mouse and human membrane proteins?

a. fluorescence microscopy with gene fusion reporters
b. fluorescence microscopy with fluorophore-labeled antibodies
c. transmission electron microscopy
d. scanning electron microscopy
e. fluorescence microscopy with fluorophore-labeled nucleic acids

Answer 1

A) fluorescence microscopy with fluorophore labeled antibodies

E) fluorescence microscopy with gene fusion reporters