Question

The nitrite ion is involved in the biochemical nitrogen cycle. You can determine the nitrite ion content of a sample using spectrophotometry by first using several reactions to form a colored compound from the ion. The following data were collected.

Nitrite Ion concentration (M) Absorbance of solution at 550 nm
2.00 x10^-6 M 0.065
6.00 x10^-6 M 0.205
10.00 x10^-6 0.338
14.00 x10^-6 0.474
18.00 x10^-6 0.598
Unknown 0.402

Required:
a. Construct a calibration plot, and determine the slope and intercept.
b. What is the nitrite ion concentration in the unknown solution?

Answer 1

Follows are the solution to the given points:

Step-by-step explanation:

In point a:

Just the y-axis graph intensity as well as the "y" absorption. Find a path or intercept utilizing Excel or simple math.

In point b:

There are two different ways:

Graphically = Then add the unknown absorbance.

Its intensity of the unknown, if they consider the y axis to pull the straight line to the line marked but instead drop it off on the x-axis.

= resolve,
`y=mx+b` from of the line even for the x variable and also get on

`\to ((y-b))/(m)= x`

Plug
`y \ value = (0.402)` and the un-concentration is also in place