Imagine you followed all steps correctly, with multiple attempts, and found that the purity of recombinant DHFR isolated through IMAC was not good enough. Hypothesize two changes to the procedure or experimental

Imagine you followed all steps correctly, with multiple attempts, and found that the purity of recombinant DHFR isolated through IMAC was not good enough. Hypothesize two changes to the procedure or experimental design that you could consider trying next to increase the purity of our DHFR sample and why you anticipate each to increase protein purity. (5pt)

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