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You are studying an enzyme that is inactivated by phosphorylation and create a mutant in which the threonine that is normally phosphorylated is replaced with glutamate. Predict the impact of this change on the activity of this enzyme. Group of answer choices

User Ucron
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1 Answer

10 votes
10 votes

Answer:

always active

Step-by-step explanation:

Phosphorylation is a posttranslational modification that consists of the addition of phosphate groups to specific amino acids on the protein. Phosphorylation acts as a molecular switch for proteins that are phosphorylated (i.e., in some situations phosphorylation acts to activate protein function, whereas in other situations phosphorylation can inactivate protein function). Phosphorylation modifies the three-dimensional structure of the protein, thereby affecting, for example, the accessibility of the active site of a phosphorylated enzyme to its substrate. Phosphorylation can occur only at the side chains of three amino acids: Serine, Threonine and Tyrosine. In this case, the enzyme is inactivated by phosphorylation on the Threonine residue, so it is expected that the mutant enzyme cannot be phosphorylated, remaining in an active state.

User William Robinson
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