Question

To assist in completing this question, you may reference the Animated Technique Video - Gel-Filtration Chromatography. Consider a mixture of four proteins with various molecular weights. A histone molecule weighs 15 kDa, a p53 molecule weighs 53 kDa, an actin molecule weighs 42 kDa, and an IgG molecule weighs 150 kDa. Arrange the molecules in order of their elution from a gel filtration column

Answer 1

IgG molecule, p53 molecule, actin molecule, histone molecule

Step-by-step explanation:

In a Gel-Filtration chromatography, the larger molecules elude faster from the gel than the small ones.

In a Gel-Filtration chromatography, there is a stationary phase and a mobile phase. The stationary phase has beads with pores. Small molecules enter the beads and descend through these beads' pores. On the other hand, large molecules do not enter the beads' pores, so they go down through the mobile phase faster than the small molecules. Since they do not have the obstacle of the pores, they descend between the beads more fluidly. As a result, the larger molecules elute first from the gel than the small molecules.