Question

What restriction enzyme would you use to cut a plasmid for insertion of the gene of interest in the creation of a recombinant plasmid?

Answer 1

Restriction endonucleases

Step-by-step explanation:

Restriction endonucleases are restriction enzymes that is used in cutting of deoxyrbonucliec acid (DNA)that recognizes a target sequence known as target site and then cuts the DNA into two pieces at or near that site.

Many restriction enzymes produce cut the ends with short, single strand, two of which if match can base pair and stick together. The enzyme DNA ligase helps in the joining of the molecule which seals gaps in the DNA backbone.