One difference between gene regulation in prokaryotes and eukaryotes is that Prokaryotes do not store their genes in a nucleus like eukaryotes do.
Option D is correct
In prokaryotes (bacteria and archaea), genetic material is not enclosed within a membrane-bound nucleus. Instead, it is located in the nucleoid region, which is a dense region within the cell. Prokaryotes have a simpler organization of their genetic material.
In contrast, eukaryotes (plants, animals, fungi, and protists) have their genetic material enclosed within a membrane-bound nucleus. The nucleus separates the transcription (the synthesis of RNA from DNA) and translation (the synthesis of proteins from RNA) processes in eukaryotic cells, allowing for more intricate control and regulation of gene expression.