Question

A solution of the iron-transport protein transferrin was titrated with a standard solution of Fe3 . The blank absorbance was 0.04, and the absorbance of 10.00 mL of the transferrin solution measured after adding 6.00 mL of titrant was 0.65. Calculate the corrected absorbance that should be plotted.

Answer 1

The corrected absorbance is calculated by subtracting the blank absorbance from the observed absorbance after titration; in this case, it is 0.61, which is the value that should be plotted.

Step-by-step explanation:

To calculate the corrected absorbance to be plotted for the titration of the iron-transport protein transferrin, you would need to subtract the blank absorbance from the observed absorbance after titration. The blank absorbance is the reading due to the instrument's background noise, container, solvent, or other factors not related to the sample's absorbance. For this example, the observed absorbance after adding the titrant to the transferrin solution is 0.65, and the blank absorbance is 0.04. So, the corrected absorbance is:

Corrected absorbance = observed absorbance – blank absorbance

Corrected absorbance = 0.65 – 0.04 = 0.61

This corrected absorbance of 0.61 is the value that should be used for plotting purposes in the titration analysis.