Final answer:
The Kirby-Bauer method of antimicrobic sensitivity testing involves inoculating the agar plate, incubating it, measuring zones of inhibition, and interpreting the results.
Step-by-step explanation:
The correct order of steps in the Kirby-Bauer method of antimicrobic sensitivity testing is:
- Inoculate the agar plate: Standardized cell suspensions are inoculated onto the surface of a specific type of medium using a sterile swab to completely cover the surface of the medium with bacterial cells.
- Incubate: The plates are incubated, usually overnight at 37°C.
- Measure zones of inhibition: After the incubation period, the disks in the lawns are examined for a zone of inhibition, and the diameter of the zone is measured using a metric ruler.
- Interpret results: The measured zone sizes are compared to a standardized chart to determine if the bacteria are resistant, susceptible, or have intermediate susceptibility to each of the antibiotics.
So, the correct order is A) Inoculate the agar plate, incubate, measure zones of inhibition, interpret results.