Question

Place the steps of the Kirby-Bauer method of antimicrobic sensitivity testing in the correct order. A) Inoculate the agar plate, incubate, measure zones of inhibition, interpret results B) Measure zones of inhibition, interpret results, incubate, inoculate the agar plate C) Interpret results, inoculate the agar plate, incubate, measure zones of inhibition D) Incubate, inoculate the agar plate, measure zones of inhibition, interpret results

Answer 1

The steps of the Kirby-Bauer method in order are: inoculating the agar plate, placing antibiotic-impregnated disks, incubating, measuring zones of inhibition, and interpreting the results. This method is used to evaluate bacterial susceptibility to drugs.

Step-by-step explanation:

The correct order of the steps for the Kirby-Bauer method of antimicrobic sensitivity testing is as follows:

1. Inoculate the agar plate with a standardized cell suspension to form a confluent lawn of bacterial colonies.
2. Place antibiotic-impregnated filter paper disks onto the inoculated agar plate.
3. Incubate the plates, typically overnight at 37°C.
4. After incubation, measure zones of inhibition (ZOI) around each disk.
5. Finally, interpret results by comparing the measured ZOI to a standard chart to determine the susceptibility or resistance of the bacteria.

Using these steps, the Kirby-Bauer disk diffusion test is a simple and rapid method to assess bacterial susceptibility or resistance to various antibacterial drugs.

Answer 2

The Kirby-Bauer method of antimicrobic sensitivity testing involves inoculating the agar plate, incubating it, measuring zones of inhibition, and interpreting the results.

Step-by-step explanation:

The correct order of steps in the Kirby-Bauer method of antimicrobic sensitivity testing is:

1. Inoculate the agar plate: Standardized cell suspensions are inoculated onto the surface of a specific type of medium using a sterile swab to completely cover the surface of the medium with bacterial cells.
2. Incubate: The plates are incubated, usually overnight at 37°C.
3. Measure zones of inhibition: After the incubation period, the disks in the lawns are examined for a zone of inhibition, and the diameter of the zone is measured using a metric ruler.
4. Interpret results: The measured zone sizes are compared to a standardized chart to determine if the bacteria are resistant, susceptible, or have intermediate susceptibility to each of the antibiotics.

So, the correct order is A) Inoculate the agar plate, incubate, measure zones of inhibition, interpret results.