Question

The investigation was done at 37°C. (ii) State one other variable that the student should have controlled during this investigation. (1)

Answer 1

One other variable that the students should have controlled during this investigation is the **concentration of trypsin**. Ensuring that the concentration of trypsin is consistent across all test tubes is important to isolate the effect of pH on the enzyme's activity. If the concentration varies, it could introduce an additional variable that might affect the results.