Prem's conclusion that the object is a living thing would likely be based on observations related to the characteristics of life. These characteristics typically include:
1. **Cellular Structure:** Prem might have observed that the object is made up of cells, which are the basic structural and functional units of life.
2. **Growth and Development:** If the object exhibited growth or showed signs of changing over time, this could be a strong indicator of a living organism.
3. **Reproduction:** Prem may have observed that the object has the ability to reproduce or generate offspring, which is a fundamental characteristic of life.
4. **Response to Stimuli:** If the object reacted or responded to external stimuli or its environment, it could suggest that it has the ability to sense and respond to changes in its surroundings.
5. **Metabolism:** Observing metabolic processes, such as the intake of nutrients or the release of waste products, would be a significant clue that the object is alive.
6. **Homeostasis:** If Prem noticed that the object maintains a stable internal environment despite external changes, it could indicate the presence of life.
7. **Adaptation:** If the object showed signs of adaptation or evolution over time to better suit its environment, this would strongly support the conclusion of life.
The specific observations that led to Prem's conclusion would depend on the details of the object and the tests he performed.