Question

Consider the data for an enzymatically catalyzed reaction. and Km from the Michaelis-Menton plot below. Work max a. Estimate the values of V, must be shown for credit. 0.6 0.5 0.4 Vo (mm/min) 0.3 0.21 0.11 0.8.5 0.5 2.0 2.5 1.0 1.5 [S] (MM) b. Discuss potential sources of error in your estimates. C. Convert the data into an Eadie-Hofstee plot and calculate Vmax and Km from the plot. d. Discuss the differences between the two and the relative sources of error.

Answer 1

Consider the data for an enzymatically catalyzed reaction. and Km from the Michaelis-Menton plot below. Work max a. Estimate the values of V, must be shown for credit. 0.6 0.5 0.4 Vo (mm/min) 0.3 0.21 0.11 0.8.5 0.5 2.0 2.5 1.0 1.5 [S] (MM) b. Discuss potential sources of error in your estimates. C. Convert the data into an Eadie-Hofstee plot and calculate Vmax and Km from the plot. d. Discuss the differences between the two and the relative sources of error.

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