To isolate intact living cells from a green leaf, the leaf is carefully harvested from a healthy plant and surface-sterilized to remove any external contaminants. Subsequently, the leaf is cut into small pieces or crushed to release the cellular content. Cell separation techniques, such as filtration or centrifugation, may be employed to isolate the intact living cells from the cell debris and other components. The resulting cell suspension can then be further analyzed, cultured, or used for various scientific investigations, providing valuable insights into plant physiology and cellular functions. It is essential to maintain sterile conditions throughout the process to ensure the viability and integrity of the isolated cells.