Question

DEAE cellulose that you'll use a cation or anion exchanger? Draw a diagram that shows the charge on the column and the charge on the sample molecules - that do and don’t bind to the column.

Answer 1

DEAE cellulose is a cation exchanger. It contains positively charged aminoethyl groups on its resin, and it interacts with negatively charged molecules in ion exchange chromatography.

Step-by-step explanation:

Column (DEAE Cellulose)

(Positively Charged Resin)

+++++++++++ +++++++++++ +++++++++++

+ + + + + +

+ + + + + +

+ + + + + +

+++++++++++ +++++++++++ +++++++++++

^ ^ ^

| | |

Negatively charged Neutral or Positively charged

molecules weakly charged molecules

molecules (do not (bind to the column)

bind to the column)