Final answer:
The anti-Western film from the 2000s influenced by Sergio Leone's Spaghetti Westerns is 'The Assassination of Jesse James by the Coward Robert Ford.' It represents a nuanced view of the Old West, moving away from exaggerated violence and myths. This echoes broader cinematic trends where technology shapes narrative complexity.
Step-by-step explanation:
An American anti-Western film from the 2000s that is influenced by the tradition of Sergio Leone's Spaghetti Westerns from the 1960s is 'The Assassination of Jesse James by the Coward Robert Ford'. This film reflects a shift from traditional portrayals of the Wild West, characterized by exaggerated violence and mayhem, towards a more nuanced and reflective iteration. It acknowledges that while there was violence in the Old West, it was often not as sensational as depicted in prior media.