The Isthmus of Panama formed about 3 million years ago, isolating the Caribbean Sea from the Pacific Ocean. Which observation would provide evidence that sister species of angelfish found on either side

The Isthmus of Panama formed about 3 million years ago, isolating the Caribbean Sea from the Pacific Ocean. Which observation would provide evidence that sister species of angelfish found on either side are the result of a vicariance event cause by the formation of the landmass, rather than the dispersal of a founder population across the Isthmus?

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