Answer:
In summary, the primer with the sequence 5’-ATTC-3’ is expected to yield the longest product during PCR, as it complements the start of the template sequence, enabling efficient amplification of a longer fragment from the beginning of the DNA template.
Step-by-step explanation:
To determine which primer will give you the longest product during PCR using the given DNA sequence as a template, we need to identify the primer that has the best complementarity with the template sequence, allowing for efficient binding and amplification.
Let's analyze the options:
5’-TTAT-3’: This primer sequence is complementary to the sequence at the end of the template (3’-AAAT-5’), allowing it to bind and initiate DNA replication. It will result in amplifying a shorter fragment from the end of the template.
5’-GGTA-3’: This primer sequence is complementary to a specific region in the template sequence (3’-CCAT-5’), allowing it to bind and initiate DNA replication. It will result in amplifying a longer fragment from the middle of the template.
5’-ATTC-3’: This primer sequence is complementary to the beginning of the template sequence (3’-TAAG-5’), allowing it to bind and initiate DNA replication. It will result in amplifying a longer fragment from the start of the template.
5’-AAAC-3’: This primer sequence is complementary to a specific region in the template sequence (3’-TTTG-5’), allowing it to bind and initiate DNA replication. It will result in amplifying a shorter fragment from the middle of the template.
5’-CTGA-3’: This primer sequence is complementary to the sequence at the end of the template (3’-GACT-5’), allowing it to bind and initiate DNA replication. It will result in amplifying a shorter fragment from the end of the template.
Based on this analysis, the primer with the sequence 5’-ATTC-3’ is likely to give you the longest product during PCR. This primer binds to the start of the template sequence, allowing for replication and amplification of a longer fragment from the beginning of the template.