Question

question at position 3 kent remembers that silica gel is acidic, so he expects the basic compound(s) to stay on the baseline of the tlc plate. however, he decides to proceed with his plan to separate them by column using the solvent system he identified by tlc. question 3. what compound will kent see elute from the column first?

Answer 1

The compound that Kent will see elute from the column first will be the most basic compound.

Step-by-step explanation:

Based on the information provided, Kent is using reverse phase HPLC to separate the compounds. In this technique, more hydrophobic compounds are attracted to the stationary phase and move more slowly through the column. Therefore, the compound that is least hydrophobic or most polar will elute from the column first. Since silica gel is acidic, the basic compound(s) will stay on the baseline of the TLC plate. Therefore, the compound that Kent will see elute from the column first will be the most basic compound.

Answer 2

To determine which compound will elute from the column first in column chromatography, we need to consider the principles of chromatography. The compound that elutes first is typically the one that interacts less strongly with the stationary phase (silica gel) and more strongly with the mobile phase (solvent). Given that silica gel is acidic and Kent expects basic compounds to stay on the baseline of the TLC plate, the acidic compound will likely elute first because it will have weaker interactions with the acidic silica gel.

Here are the steps to understand this process:

Step 1: Understand the principles of column chromatography.

- Column chromatography separates compounds based on their differential adsorption and desorption on a stationary phase (silica gel) and their movement through a mobile phase (solvent).

Step 2: Consider the nature of the compounds.

- Silica gel is acidic, so it tends to interact more strongly with basic compounds (compounds that can accept protons, such as amines).

- The basic compound will have stronger interactions with the silica gel, causing it to move more slowly through the column.

Step 3: Determine the order of elution.

- Since the basic compound interacts more strongly with the silica gel, it will have a slower rate of migration through the column.

- The acidic compound, which interacts less strongly with the silica gel due to its acidic nature, will elute first because it moves more easily through the column.

So, Kent will see the acidic compound elute from the column first.