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16. The method of movement that requires the expenditure of ATP molecules is

a simple diffusion

c. Osmosis

b. Facilitated diffusion

d. Passive transport

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Answer: Active Transport

Explanation: Active transport is the movement of molecules or ions across the cell membrane against a concentration gradient, requiring the use of energy in the form of ATP. This technique allows cells to move substances from lower concentration to higher concentration areas, which is the opposite direction of passive transport ( simple diffusion, osmosis or facilitated diffusion).

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