Question

If I was to grow a nitrifying archaea that was from a nearby lake, and was using a broth for the media what exactly would I need to add to the broth to make sure it grew well? If it needs carbon what would I add to the broth?  If I needed nitrogen what would I add to the broth? If I needed anyother nutrents what would they be?

Answer 1

Answer and Explanation:

Nitrifying archaea, also known as ammonia-oxidizing archaea (AOA), are microorganisms involved in the biogeochemical cycling of nitrogen. To grow AOA from a nearby lake in a broth media, you would need to provide the necessary nutrients and conditions for their growth.

If the AOA require carbon, you could add a carbon source such as glucose or acetate to the broth. If they require nitrogen, you could add a nitrogen source such as ammonium or nitrate to the broth. Other nutrients that may be required for the growth of AOA include phosphorus, sulfur, and trace minerals.

It’s important to note that routine cultivation of AOA is typically performed in liquid cultures, and their growth requirements may vary depending on the specific strain and environmental conditions1. It may be helpful to consult the scientific literature or seek guidance from an expert in the field to determine the optimal growth conditions for the specific strain of AOA you are trying to cultivate.