Answer: The most likely explanation for the allele differences observed in Greater Prairie Chickens between the 1950s and 2000s is genetic bottleneck. This occurs when a population experiences a significant reduction in its total size, resulting in a decrease in the amount of genetic diversity within the population. This can lead to a decrease in the number of alleles per gene locus, as individuals may carry the same alleles more frequently due to the reduced gene pool. In the case of Greater Prairie Chickens, it is possible that the population experienced a genetic bottleneck in the late 20th century, resulting in a loss of genetic diversity and a decrease in the number of alleles per gene locus in contemporary samples compared to historic samples.