Answer:
To draw the other strand of the DNA molecule, you need to identify the complementary bases for each base in the given sequence. In DNA, adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G). By applying these base-pairing rules, we can determine the complementary strand:
Given sequence (5' - CCTTGACGATCGTTACCG-3')
Complementary strand (3' - GGAACTGCTAGCAATGGC-5')
To determine if the Pvul restriction enzyme will cut this DNA molecule, we need to look for the recognition sequence of the enzyme, which is the specific DNA sequence it recognizes and cuts. The recognition sequence for Pvul is 5' - CGATCG - 3'.
Looking at the complementary strand, we can see that the recognition sequence (CGATCG) is present in the given DNA sequence. Therefore, Pvul will cut this DNA molecule.
When Pvul cuts DNA, it creates "sticky ends" or overhanging ends that are complementary to each other. In this case, Pvul cuts between the second and third base pairs of the recognition sequence (CGATCG) on both strands, resulting in the following products:
5' - CCTTGACGATC - 3'
3' - GGAAC T GCTAG - 5'
Note that the enzyme cuts between the G and A on the top strand and between the C and T on the bottom strand. The resulting products have overhanging ends: 5' - GATC - 3' on the top strand and 3' - CTAG - 5' on the bottom strand, which can potentially bind with each other.
Step-by-step explanation:
with this explanation i hope now you are able to draw