Answer:
When using an oil immersion lens objective, the objective lens and the specimen should be immersed in a transparent oil of high refractive index, typically with a refractive index of around 1.515. The amount of oil required is subjective and depends on the specific lens being used. It is advised to use only enough oil to fill the gap between the objective lens and the slide, without excess oil spilling over the edges of the coverslip. The oil should be applied directly onto the coverslip and the objective lens should be slowly lowered into the oil, allowing the oil to come into contact with the slide. Using too much oil can result in image distortion, while using too little oil will not provide the increase in resolution desired. It is important to only use immersion oil with an immersion objective lens designed for this purpose, as attempting to use immersion oil with a "dry" objective lens will only foul the lens
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