Answer:
1. Enhancement of existing laboratory techniques
a. Sanger sequencing
b. Large-scale DNA sequencing
2. Comprehensive understanding of biological phenomena
a. Gaucher Disease
b. Spike Proteins
c. Neurodegenerative Diseases
3. Development of new technologies
4. Creation of new therapeutic approaches
a. Therapeutic Proteins
b. Gene Therapy
5. Creation of characteristics not found in nature
a. Taliglucerase alfa
b. Golden Rice
Step-by-step explanation:
1. Enhancement of existing laboratory techniques
Genetic engineering is not a stand-alone technology, but it has improved and enhanced existing laboratory techniques. DNA sequencing technology is one of the laboratory techniques that became more powerful when recombinant DNA technology was incorporated into its workflow.[2]
For instance:
1.1. Sanger sequencing
Sanger sequencing is a chain-termination DNA sequencing technique that uses dideoxynucleotides to terminate the synthesizing DNA chains. At the start of the reaction, oligonucleotides anneal to the complementary DNA sequences before the chain synthesis and termination occur.
The priming of oligonucleotides limits the sequencing of DNA fragments to only those with partially known DNA sequences. With molecular cloning, the ligation.