The exclusion limit of a SEC is 25 kD, if you apply a mix protein extract containing following molecules, (moleculet: MW 32,000 D Molecule 2: 10 KD, molecule3: 10,000 D and molecule 4:45 KD At the end of elution 1-how many fractions you will get, briefly explain why 1 2-which molecule/s will be fractionated and which will be excluded