133k views
1 vote
list the steps of the gram stain in appropriate order and describe what is happeningat each step in the process

1 Answer

5 votes

Answer:

The Gram stain is a laboratory test that checks for bacteria at the site of a suspected infection or in certain bodily fluids. It is a differential method of staining used to assign bacteria to one of two groups (gram-positive and gram-negative) based on the properties of their cell walls.

The gram staining procedure involves four major steps:

1. Staining with crystal violet - Flood air-dried, heat-fixed smear of cells for 1 minute with crystal violet staining reagent.

2. Fixing the dye - Add a few drops of Gram’s iodine (mordant) which forms a complex with crystal violet.

3. Applying a decolorizer - Rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone.

4. Counter-staining - Counterstaining with safranin

User Ankit Gupta
by
8.5k points

No related questions found