In a ChIP-seq experiment using an antibody recognizing the Gal4 protein in Saccharomyces cerevisiae, you would expect to recover DNA sequences containing Gal4 binding sites. Gal4 is a transcription factor that regulates the expression of genes involved in galactose metabolism, specifically the GAL genes.
The Gal4 protein recognizes and binds to a specific DNA sequence called the UAS_G (Upstream Activating Sequence for GAL genes), which is found upstream of the target genes in the yeast genome. The UAS_G consensus sequence is typically 17 base pairs long and contains a palindromic core sequence, CGG-N11-CCG.
Upon performing ChIP-seq, the recovered DNA sequences would likely contain UAS_G sequences along with nearby genomic regions. This is because the chromatin fragments generated during the experiment may include additional nucleotides adjacent to the binding sites. Analyzing these sequences can provide insight into the genomic locations where Gal4 interacts with chromatin, revealing potential regulatory roles for Gal4 in gene expression and galactose metabolism.
In summary, by extracting chromatin from Saccharomyces cerevisiae cells and performing a ChIP-seq experiment with an antibody against the Gal4 protein, you would expect to recover DNA sequences containing Gal4 binding sites, primarily consisting of UAS_G sequences and adjacent genomic regions.