Question

Which of the following tools of DNA technology is incorrectly paired with its use?

(a) DNA polymerase chain reaction to amplify segments of DNA
(b) DNA ligase cutting DNA, creating sticky ends of restriction fragments
(c) Electrophoresis separation of DNA fragments

Answer 1

Biology

Step-by-step explanation:

The incorrect pairing of a tool of DNA technology with its use is:

(b) DNA ligase cutting DNA, creating sticky ends of restriction fragments.

The correct pairing should be:

(b) DNA ligase joining DNA fragments together by catalyzing the formation of phosphodiester bonds between their sugar-phosphate backbones. DNA ligase is commonly used in recombinant DNA technology to create recombinant DNA molecules. It is not involved in cutting DNA or creating sticky ends of restriction fragments. That role is performed by restriction enzymes.