Final answer:
Evidence against preformationism includes the role of zygotically expressed and maternal-effect genes in regulating sequential and complex embryonic development, as well as the interaction of genes during the processes of body axes formation and organogenesis.
Step-by-step explanation:
The hypothesis of preformationism, which suggested the presence of a miniature adult or homunculus within the sperm or egg that would simply grow during development, is disproven by various pieces of evidence that emphasize the complexity and sequential nature of embryonic development. One of the key pieces of evidence against preformationism includes the zygotically expressed genes that regulate development in a sequential manner. Another important aspect is the influence of maternal-effect genes on embryonic development, which can determine the outcome of the embryo before zygotic genes start being expressed.
The discovery that genes expressed in the developing embryo interact with each other in a highly intricate and organized manner also refutes preformationism. The fact that specific genes are activated at certain times during development and can cause cells to differentiate into various types translates into complex development far beyond the concept of a preformed homunculus. Furthermore, experiments with model organisms such as the fruit fly (Drosophila) have shown that embryonic development involves the formation of body axes and organogenesis, guided by the expression of certain genes, rather than the expansion of a preformed structure.