Question

Based on the plasmid that will be added to each sample, fill in the sgRNA and Donor DNA columns with "+" or "–" to indicate which components those bacteria will have.

Answer 1

The '+' or '–' in the sgRNA and Donor DNA columns represent the presence or absence of these components in the bacteria. sgRNA directs gene editing, while Donor DNA serves as a genetic template in the transformation process using plasmids.

Step-by-step explanation:

The student's question pertains to a laboratory procedure involving the transformation of E. coli cells with plasmids that contain components such as sgRNA and Donor DNA for genomic engineering purposes. When these transformed cells are cultured on a medium containing an antibiotic like ampicillin, only those cells that have successfully taken up the plasmid will grow, as the plasmid confers antibiotic resistance. The sgRNA component is typically used for targeted gene editing, while Donor DNA provides a template for repair or insertion of new genetic material.

In the provided context, '+ ' in the sgRNA and Donor DNA columns would indicate the presence of these components in the bacteria, whereas a '–' would indicate their absence. Since the specific experimental design is not provided, the student should use the principles discussed, where the presence of sgRNA implies targeted gene editing and Donor DNA indicates the template for genetic insertion during the transformation process.