Question

What steps in the eukaryotic transcription cycle are stimulated by phosphorylation of the carboxyl terminal (CTD) of the large subunit of RNA polymerase II and beyond?

Check all that apply.

promoter escape

a. efficient termination

b. efficient elongation

c. recruitment of factors required for initiation

d. recruitment of factors required for RNA processing

Answer 1

Phosphorylation of the carboxyl terminal domain (CTD) of the large subunit of RNA polymerase II is important for the regulation of transcription in eukaryotes. The phosphorylation of CTD is required for several steps in the transcription cycle, including promoter escape and efficient elongation. Therefore, the correct options are:

a. promoter escape
b. efficient elongation

Option c is incorrect because the recruitment of factors required for initiation is stimulated by the phosphorylation of the CTD of the small subunit of RNA polymerase II. Option d is incorrect because the recruitment of factors required for RNA processing is not directly stimulated by the phosphorylation of the CTD of RNA polymerase II.