Darwin's argument that vestigial structures are evidence that evolution has occurred was not used to develop the scientific theory of how life began on Earth. The other three options are all relevant to the development of this theory. Oparin's theory proposed that the first living organisms arose from a mixture of organic compounds in the Earth's early oceans, and Miller and Urey's experiment supported this idea by demonstrating that these compounds could be formed through natural processes. Pasteur's experiments demonstrated that life does not arise spontaneously, which helped to reject the idea of spontaneous generation and support the theory of biogenesis. Margulis proposed the endosymbiotic theory, which explains the origin of eukaryotic cells and their organelles, and this theory is supported by scientific evidence.