If the colony of viruses quadruples its number every second, then the number of viruses in the previous second must have been one-fourth of the number of viruses at the particular time when the number was 655,360.
We can find the number of viruses in the previous second by dividing 655,360 by 4:
655,360 ÷ 4 = 163,840
Therefore, there were 163,840 viruses in the previous second.